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dc.contributor.authorGonenc, G.
dc.contributor.authorIsci, H.
dc.contributor.authorYigiter, A. B.
dc.contributor.authorHancer, V.
dc.contributor.authorBuyukdogan, M.
dc.contributor.authorGuducu, N.
dc.contributor.authorDunder, I.
dc.date.accessioned2019-08-13T12:10:23Z
dc.date.accessioned2019-08-13T16:02:21Z
dc.date.available2019-08-13T12:10:23Z
dc.date.available2019-08-13T16:02:21Z
dc.date.issued2015
dc.identifier.issn0390-6663
dc.identifier.urihttp://hdl.handle.net/11446/2783
dc.descriptionWOS: 000356108200017en_US
dc.descriptionPubMed ID: 26152007en_US
dc.description.abstractObjective: Anti-D immunoglobulin is applied to all pregnant women having Rh]) incompatibility to prevent hemolytic disease of the newborn. The aim of this study is to determine fetal RE]) status in the Rh incompatible pregnancies with an non-invasive technique; free fetal DNA isolation from maternal circulation. In the case of Rh incompatibility especially with a history of previous fetal anemia, it can be beneficial to know Rh status antenatally in terms of monitoring fetuses with Rh positive [RhD(+)] status consciously. Materials and Methods: Total free DNA was isolated in 50 Rh negative [RhD()] pregnant women, who had Rh]) alloimmunisation with their husbands. The gene in isolated DNA was investigated with TagMan prob and real time PCR by using primers belonging to exon 7 of the RhD gene. Results: The authors analyzed 50 RhD() women by using quantitative real time PCR technique. Five of them were RhD() and the rest of them were found to be RhD(+). After birth one of the infants who were analyzed as RhD(+) were found to be RED(). Conclusion: The detection of fetal RED status by using a non-invasive method from maternal circulation was found to be possible. Assessing fetal RED status non-invasively by using free fetal DNA in maternal blood will be cost-efficient, avoiding unnecessary indirect Coombs test and unnecessary Rhogam applications that is used in RH incompatible pregnancies. This study will throw a fresh light on prenatal diagnosis.en_US
dc.language.isoengen_US
dc.publisherI R O G CANADA, INCen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPrenatal diagnosisen_US
dc.subjectFetal DNAen_US
dc.subjectRED geneen_US
dc.titleNon-invasive prenatal diagnosis of fetal RhD by using free fetal DNAen_US
dc.typearticleen_US
dc.relation.journalCLINICAL AND EXPERIMENTAL OBSTETRICS & GYNECOLOGYen_US
dc.contributor.departmentDBÜen_US
dc.identifier.issue3en_US
dc.identifier.volume42en_US
dc.identifier.startpage344en_US
dc.identifier.endpage346en_US
dc.contributor.authorID0000-0003-2994-1077en_US
dc.contributor.authorID0000-0001-7948-0235en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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